Ian Marshall, Susan Al-Kazwini, Jenny J. Holman, Peter M.'s Neuroendocrine Molecular Biology PDF


By Ian Marshall, Susan Al-Kazwini, Jenny J. Holman, Peter M. Broad, Mark R. Edbrooke (auth.), G. Fink, A. J. Harmar, Kenneth W. McKerns (eds.)

ISBN-10: 1468451316

ISBN-13: 9781468451313

ISBN-10: 1468451332

ISBN-13: 9781468451337

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Terenius, L. and Bloom, F. , 1982 Dynorphin is contained within hippocampal mossy fibers: immunochemical alterations after kainic acid administration and colchicine-induced toxicity, Proc. Natl. Acad. Sci. USA 79:6747-6751. McLaughlin, B. , Wood, J. , Saito, K. , Vaughn, J. E. Roberts, E. and Wu, J. , 1974 The fine structural localization of glutamate decarboxylase in synaptic terminals of rodent cerebellum, Brain Res. 76:377-391. Milner, R. J. and Sutcliffe, J. , 1984 Gene expression in rat brain, Nuc.

In our studies we have used 32p labelled cDNA or synthetic oligonucleotide probes. Whilst 32p labelling does not provide the resolution of 3H, 35S or 125 1 labelling when steps are taken to minimize the problem of 32p adequate resolution for our needs down to a single cell can be obtained. 33 32P-labelling of probes CDNA nick translation random primers riboprobe SP6 Synthetic DNA S' with polynucleotide kinase DNA polymerase 3 DNA polymerase is used also to incorporate nucleotide labelled with H, 3SS and 12SI.

A. Higgins is supported by an NIH NRSA award No. 5 F32 NS07528-02. We wish to thank Peggy Graber for assistance in preparing the manuscript. 29 REFERENCES Amara, S. , Rosenfeld, M. , Ong, E. S. and Evans, R. , 1982 Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products, Nature 298: 240-244. -Bantle, J. A. and Hahn, W. , 1976 Complexity and characterization of polyadenylated RNA in the mouse brain, Cell 8:193-150. Beckman, S. , Chikaraishi, D.

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Neuroendocrine Molecular Biology by Ian Marshall, Susan Al-Kazwini, Jenny J. Holman, Peter M. Broad, Mark R. Edbrooke (auth.), G. Fink, A. J. Harmar, Kenneth W. McKerns (eds.)

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