Frost, Drought, and Heat Resistance - download pdf or read online
By J. Levitt (auth.)
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Additional resources for Frost, Drought, and Heat Resistance
The older experiments failed to result in more than a very slight increase in frost resistance. But this was due tq the fact that very frost sensitive plants were used-plants normally completely killed by - 2 to -3 0 C. (LEVITT 1956). Failure to protect these plants is to be expected, since the osmotic protection is proportional to the original frost resistance. When plants that are already somewhat frost resistant are used, very significant increases in resistance result fr()lm treatment with penetrating solutes-increases that are precisely what would be expected from a purely osmotic effect (LEVITT 1957 b).
In two. cases, sprays have been asserted to. increase hardiness. CORNS and SCHWERDTFEGER (1954) grew beet seedlings in vermiculite moistened with (a) water, (b) 4-8 ppm. piDnic acid. The shoots were then severed and exposed to. -100 C. for 5-10 mins. A highly significant increase in survival was Dbtained in the case Qf the chemically treated. This, Df CDurse, yields no. rt to. permit freezing equilibrium, if freezing Dccurred at all. It may pDssibly be a case Df ('hilling injury, Dr perhaps frost aVDidance, due to.
This was proved by ultrarapid freezing to -160° C. in a bath of dichlorQodi£luQoromethane followed by transfer to an alcohol bath at -600 C. which was heated at a rate of 1o/min. Tubes removed at -410 C. showed 20% hemolysis; those removed at -390 C. and similarly thawed showed 80% hemolysis. Within the critical temperature region, the amount of hemolysis varied with the speed of freezing and thawing (Table 11). It is interesting to note that in the case Qof the bacterium Pasteurella Tulariensis, the critical region is -30 to -450 C.
Frost, Drought, and Heat Resistance by J. Levitt (auth.)