Methods in Microbiology, Vol. 13 by T. Bergan, J.R. Norris (Eds.) PDF


By T. Bergan, J.R. Norris (Eds.)

ISBN-10: 0125215134

ISBN-13: 9780125215138

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Additional resources for Methods in Microbiology, Vol. 13

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1926) which reacted quite differently. Similar results were obtained by Carey (1936). Webb and Barber (1937) noted a great similarity in the serological behaviour of Listeria strains tested by them. Schultz, Terry, Brice and Gebhardt (1938) as well as Julianelle and Pons (1939) first drew the conclusion that Listeria should be divided into two distinct serological groups, which seemed to be connected with the origin of the strain. In consequence a “rodent group” and a “ruminant group” were distinguished.

The supernatant is removed and the bacteriocin is precipitated by the addition of powdered ammonium sulphate to 40% saturation (28 g/100 ml) at 4°C. The fluid is constantly stirred by a magnetic stirrer during this procedure. Good yields of bacteriocin are attainable in 1-2 h, although we usually allow precipitation to continue overnight. The precipitate is collected by centrifugation at 6000 x g for 10 min and resuspended in 5 ml of sterile Brain Heart Infusion Broth. Buffers might be substituted for'broth, although we have found broth preferable for preserving bacteriocin activity.

Novyi types A and B was influenced by bacteriophages. When the type A organism lost its phage, the resulting non-toxigenic bacterium more closely resembled C. botulinum types C and D than the other types of C. novyi. B. Bacteriophages of other clostridia 1. Bacteriophages of C . botulinum Bacteriophages are commonly associated with C. botulinum and phages may, at least in some cases, govern toxin production. In 1968, Vinet et al. briefly described a phage of C. botulinum type C . Inoue and Iida (1968) described phages induced from strains of C.

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Methods in Microbiology, Vol. 13 by T. Bergan, J.R. Norris (Eds.)

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