Virus-Resistant Transgenic Plants: Potential Ecological - download pdf or read online
By Mark J. Gibbs, John Armstrong (auth.), Dr. Mark Tepfer, Dr. Ervin Balázs (eds.)
The advent of novel genes into crops through genetic transformation holds nice promise for plant breeding, and lots of crop species were rendered virus-resistant by way of expression of viral sequences. despite the fact that, it's necessary to additionally assessment the aptitude dangers linked to this new expertise. one of the kinds of genetically transformed vegetation which could signify capability ecological dangers, ones expressing viral sequences pose questions of specific curiosity. during this quantity designated recognition is given to recombination in vegetation expressing sequences of RNA or DNA viruses, heterologous encapsidation or other kinds of complementation in vegetation expressing coat protein genes, strength deleterious results of satellite tv for pc RNAs linked to cucumber mosaic virus, and sexual transmission of virus resistance genes to probably weedy relations.
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Additional resources for Virus-Resistant Transgenic Plants: Potential Ecological Impact
Future experiments will be aimed at describing the function and structure of putative recombinosomes in both the homologous and nonhomologous systems in BMV. Also, the molecular components of the recombinosomes will be defined biochemically. SimiJar studies with other RNA viruses are needed to elucidate the general or unique nature of BMV recombination. Acknowledgments This research was supported by grants from NIH ( ROI AI26769 and 3ROI AI26769), NSF (MCB-9305389 and MCB-9630794), and by the Plant Molecular Biology Center at Northem Illinois University.
Virus Genes 9:193-195 Keese P, Symons RH (1985) Dornains in viroids: evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proc Natl Acad Sei USA 82:4582-4586 Kerlan C, Tribodet M, Glais L, Astier-Manifacier S, RobagIia C (1997) Polymorphisme de la region 5' de I'ARN viral et criteres de differenciation des souches du virus Y de la pomme de terre. VI cmes Rencontres de Virologie Vegetale CNRS-INRA. p5 Maynard-Srnith I (1992) Analyzing the mosaic structure of genes.
Assuming coordinated evolution of all parts of the genome, aIJ reconstruetions should show simiJar topologies. Any shift in the affinities of a given isolate is thus likely to refleet the influence of recombination events. The recombinations thus deteeted were then validated using statistical analysis. A similar approach allowed Chenault & Melchers (1994) to demonstrate tbe frequent occurrence of recombinant isolates in aplant pararetrovirus, cauJiflower mosaie virus. , 1996). In the case of eueumber mosaic eucumovirus (CMV) all fuIJ-length sequences of genomic RNAs were extracted from databanks and analysed : 012537, MCVFRNA1, MCVL1, CMU20220, MCV1A1 and CURNA1Q (RNAI); MCVRN2, MCVORNA2, CMU66287, CMU59740, MCVL2, 086613, S72187, MCV2A2, 086330, 012538, CMU20218 and CVRNA02 (RNA2); and MCVRNA3, MCVRNA3A, MCV3APCOAT, CMV3ACP, MCVCMVD, MCV3A3, MCVCMVA, MCVPT7CKY3, 22 MCVYRNA3, MCVR3MPCP1, MCV3ACP, MCVCP3A2, MCVCMVC, MCVR3MPCP2, MCVCMVB, MCVL3, MCVCP3A1, CMU66094, MCV03, MCVRNA3KOR and CMU20219 (RNA3).
Virus-Resistant Transgenic Plants: Potential Ecological Impact by Mark J. Gibbs, John Armstrong (auth.), Dr. Mark Tepfer, Dr. Ervin Balázs (eds.)