Download PDF by Ju Yan, Macoura Gadji, Kada Krabchi, Régen Drouin (auth.),: PRINS and In Situ PCR Protocols


By Ju Yan, Macoura Gadji, Kada Krabchi, Régen Drouin (auth.), Franck Pellestor (eds.)

ISBN-10: 1588295494

ISBN-13: 9781588295491

ISBN-10: 1597450685

ISBN-13: 9781597450683

Carrying at the excessive criteria of the much-praised first variation, major researchers and medical investigators have thoroughly revised and up to date PRINS and In Situ PCR Protocols to use the numerous new advancements and functions that experience happened for mobile and molecular investigations. The authors give a contribution confirmed protocols for lots of adaptations of PRINS and in situ PCR, together with a brand new, quick multicolor PRINS strategy for picking human chromosomes, PRINS protocols for in situ detection of precise sequences and aspect mutations, a brand new dideoxy PRINS process, and in situ PCR and PRINS protocols for crops. They describe, intimately, quite a few functions of PRINS in human cells (detection of gene deletions in melanoma, detection of fetal cells in maternal blood, and overview of aneuploidy in mind tissues and embryos) in addition to in plant cells. with no trouble reproducible in situ PCR innovations also are awarded for the detection of cytomegalovirus and to be used together with microdissection. In situ RT-PCR strategies also are given to be used in plant or melanoma research. The protocols stick to the winning tools in Molecular Biology™ sequence structure, every one supplying step by step laboratory directions, an advent outlining the rules at the back of the procedure, lists of the required gear and reagents, and pointers on troubleshooting and keeping off identified pitfalls.
complete and hugely functional, PRINS and In Situ PCR Protocols bargains molecular biologists, cytogeneticists, and cytologists a state of the art advisor to modern-day new in situ labeling technology.

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9. , and Norden, B. (2000) Peptide nucleic acid (PNA): its medical and biotechnical applications and promise for the future. FASEB J. 14, 1041–1060. 10. M. (2001) Multicolor fluorescence in situ hybridization with peptide nucleic acid probes for enumeration of specific chromosomes in human cells. Gen. Chrom. Cancer. 30, 57–63. 11. , and Williams, B. (2003) PNA on human sperm: a new approach for in situ aneuploidy estimation. Eu. J. Hum. Genet. 11, 337–341. 12. , and Pellestor, F. (2004) The peptide nucleic acids as probes for chromosomal analysis: application to human oocytes, polar bodies and preimplantation embryos.

Store at room temperature and prepare a fresh mixture every 7 d. 8. Fixative solution: 3:1 methanol:glacial acetic acid. 2. Collection of Cells and Preparation of Slides 1. Colcemid (Gibco Invitrogen). Purchased in lyophilized form, stored at 4°C and used at room temperature after reconstitution in distilled water. Discard after 1 mo. 2. Methanol (Fischer Scientific). 3. Glacial acetic acid (Fischer Scientific). 4. Glass slides and cover slips (Fisher Scientific). 3. Primed In Situ Hybridization 1.

Sci. USA 78, 6299–6303. 11. Choi, E. , Nichol, J. , Hokom, M. , Hornkohl, A. , and Hunt, P. (1995) Platelets generated in vitro from proplatelet-displaying human megakaryocytes are functional. Blood 85, 402–413. 12. Koch, J. , Petersen, K. , and Bolund, L. (1989) Oligonucleotide-priming methods for chromosome-specific labeling of alpha satellite DNA in situ. Chromosoma 4, 259–265. 13. , and Charlieu, J. P. (1995) Selection of chromosome-specific primers and their use in simple and double PRINS techniques for rapid in situ identification of human chromosomes.

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PRINS and In Situ PCR Protocols by Ju Yan, Macoura Gadji, Kada Krabchi, Régen Drouin (auth.), Franck Pellestor (eds.)

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