Download e-book for iPad: Microbial Gene Essentiality: Protocols and Bioinformatics by Karen Joy Shaw (auth.), Andrei L. Osterman, Svetlana Y.


By Karen Joy Shaw (auth.), Andrei L. Osterman, Svetlana Y. Gerdes (eds.)

ISBN-10: 1588293785

ISBN-13: 9781588293787

ISBN-10: 1597453218

ISBN-13: 9781597453219

Microbial Gene Essentiality: Protocols and Bioinformatics incorporates a complete selection of experimental and computational techniques and strategies for microbial genome-scale essentiality reviews, constructed and offered by way of the prime teams within the box. as well as wet-lab protocols, the booklet describes (i) statistical equipment crucial for making plans winning large-scale essentiality displays, in addition for info overview and research; (ii) in-silico prediction of gene essentiality utilizing genome-scale reconstructed metabolic versions; and (iii) info integration and comparative research within the context of genomic databases. This quantity presents researchers with a first-stop advisor for selecting the main applicable approach for deliberate essentiality reviews. Experimental and computational elements are both very important in genome-scale gene essentiality research, as in all different genomic applied sciences, and Microbial Gene Essentiality: Protocols and Bioinformatics displays either one of those points. All protocols stick to the winning equipment in Molecular Biology™ sequence layout, each one supplying an creation outlining the rules at the back of the options, step by step directions, lists of the mandatory gear and reagents, and tips about troubleshooting and heading off pitfalls, and are meant for either beginner and professional scientists.

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Extra resources for Microbial Gene Essentiality: Protocols and Bioinformatics

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4. Clone PCR product into the SapI sites of pXT10. 5. Transform resultant construct into H. influenzae by natural transformation (25) and select for transformants on sBHI agar containing tetracycline overnight at 35°C. 6. Disrupt or delete native copy of the essential gene of interest. This can be achieved by transforming H. influenzae with a deletion construct or a PCR product containing an antibiotic marker flanked by homologous sequences corresponding with the locus targeted for deletion. 7.

2004) 1. Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186, 4921–4930. 2 Glass, J. , Lewis, M. , et al. 2. (2006) Essential genes of a minimal bacterium. Proc. Natl. Acad. Sci. A. 103, 425–430. 3 Liberati, N. , Urbach, J. , Lee, D. , et al. (2006) 3. An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc. Natl. Acad. Sci. A. 103, 2833–2838. , and Yukawa, H. (2006) High4. throughput transposon mutagenesis of Corynebacterium glutamicum and construction of a single-gene disruptant mutant library.

DNA Res. 12, 103–115. 6 Sassetti, C. , Boyd, D. , and Rubin, E. J. (2001) Comprehensive identification of 6. conditionally essential genes in mycobacteria. Proc. Natl. Acad. Sci. A. 98, 12712– 12717. , Campbell, J. , Kay, K. , Wanner, B. , Gerdes, S. , et al. 7. (2004) Genome-scale identification of conditionally essential genes in E. coli by DNA microarrays. Biochem. Biophys. Res. Commun. 322, 347–354. 8 Gerdes, S. , Scholle, M. , Campbell, J. , Daugherty, M. , 8. et al. (2003) Experimental determination and system level analysis of essential genes in Escherichia coli MG1655.

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Microbial Gene Essentiality: Protocols and Bioinformatics by Karen Joy Shaw (auth.), Andrei L. Osterman, Svetlana Y. Gerdes (eds.)

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