N. Harris (editor), D. G. Wilkinson (editor)'s In Situ Hybridisation: Application to Developmental Biology PDF
By N. Harris (editor), D. G. Wilkinson (editor)
Advances in our figuring out of organic mechanisms have usually been linked to the advance of recent innovations. In situ hybridization is a vintage case of simply such an improve. The strategy successfully combines histochemistry with molecular biology and allows the speedy research of the distribution of RNA, or DNA, within the tissues. the data received from this has brought on whatever of a revolution in our realizing of developmental biology, when you consider that a basic point of improvement is the spatial and temporal expression of genes. moreover the approach has chanced on program within the box of drugs, the place it's giving new insights into the functioning of fit tissues and the prognosis and research of illnesses. This publication brings jointly contributions from leaders within the software of this method and should aid the reader assessment and be guided during the a number of concepts and adaptations of the method.
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Extra info for In Situ Hybridisation: Application to Developmental Biology and Medicine (Society for Experimental Biology Seminar Series (No. 40))
Brigati et al. (1983) using biotinylated DNA probes labelled by nick translation detected parvovirus, polyomavirus, herpes simplex virus and adenovirus in cultured mouse cells and human autopsy material. , 1986) have been reported. The use of biotinylated probes in viral diagnostic studies may reflect the clinicians' desire for rapid results and also the applicability of non-radioisotopic techniques to routine laboratories. High titres of viral particles are often found within infected cells, hence localisation is not hindered by the lower sensitivity of non-radiolabelled probes.
Christodoulou, C. & Gait, M. J. (1988). In situ hybridization histochemistry: localization of vasopressin mRNA in rat brain using biotinylated oligonucleotide probe. Brain Research, 464 (1), 63-9. Bannerjee, D. & Pettit, S. (1984). Endogenous avidin-binding activity in human lymphoid tissue. Journal of Clinical Pathology, 37, 223-5. Bauman, J. , Wiegant, J. & van Duijn, P. (1981). Cytochemical hybridization with fluorochrome-labeled RNA. Journal of Histochemistry and Cytochemistry, 29, 238-40. , Flannery, D.
G. (1985). Mapping muscle protein genes by in situ hybridization using biotin-labelled probes. The EMBO Journal, 4, 2493-8. Ambros, P. , Matzke, M. A. & Matzke, A. J. M. (1986). Detection of a 17 kb unique (T-DNA) in plant chromosomes by in situ hybridization. Chromosoma, 94, 11-16. Appels, R. (1989). Three dimensional arrangement of chromatin and chromosomes: old concepts and new techniques. Journal of Cell Science, 92, 325-8. Bauman, J. G. , Borst, P. & van Duijn, P. (1980). A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochrome labeled RNA.
In Situ Hybridisation: Application to Developmental Biology and Medicine (Society for Experimental Biology Seminar Series (No. 40)) by N. Harris (editor), D. G. Wilkinson (editor)