Download e-book for kindle: Genetic Recombination: Reviews and Protocols (Methods in by Alan S. Waldman


By Alan S. Waldman

ISBN-10: 1588292363

ISBN-13: 9781588292360

Genetic recombination is any strategy during which DNA sequences have interaction and endure a move of knowledge, generating new "recombinant" sequences that include details from all the unique molecules. This ebook provides major peer-reviewed protocols to hold out recombinant investigations within the lab. half I presents ways and version platforms for learning a number of elements of recombination in various eukaryotic organisms and in mammalian parasites. half II describes methods for utilizing recombination as a reporter of genomic instability in reduce and better eukaryotes. half III discusses quite a few tools and methods for precise genomic manipulation in greater and decrease eukaryotes, whereas the ultimate half offers biochemical analyses important for furthering the certainty of recombination mechanisms, hence making this a useful booklet for either the beginner and the proven researcher within the box of recombination.

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Additional info for Genetic Recombination: Reviews and Protocols (Methods in Molecular Biology Vol 262)

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Cho, J. , Khalsa, G. , and Nickoloff, J. A. (1998) Gene conversion tract directionality is influenced by the chromosome environment. Curr. Genet. 34, 269–279. 31. , Kolodkin, A. , and Stahl, F. W. (1988) Intrachromosomal gene conversion induced by a DNA double-strand break in Saccharomyces cerevisiae. J. Mol. Biol. 201, 247–260. 32. Dronkert, M. L. , Beverloo, H. , Johnson, R. , Hoeijmakers, J. H. , and Kanaar, R. (2000) Mouse RAD54 affects DNA double-strand break repair and sister chromatid exchange.

Proc. Natl. Acad. Sci. USA 85, 6022–6026. , et al. (1986) Universal code equivalent of a yeast mitochondrial intron reading frame is expressed in E. coli as a specific double strand endonuclease. Cell 44, 521–533. , et al. (1991) Cleavage of yeast and bacteriophage T7 genomes at a single site using the rare cutter endonuclease I-SceI. Nucleic Acids Res. 19, 189–190. , and Hohn, B. (1993) Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a sitespecific endonuclease.

Two groups have developed plasmids containing T. brucei sequences that are not obviously replication-related but nevertheless promote episome maintenance (20,21), and one has been used to demonstrate the essentiality of a gene via complementation (22). Artificial, linear extrachromosomal episomes have also been described for T. brucei, but these too are lost during cell divisions in the absence of drug selection (23,24). Episomal plasmid transformation remains the exception in T.

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Genetic Recombination: Reviews and Protocols (Methods in Molecular Biology Vol 262) by Alan S. Waldman

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