Rita R. And R. Grigorova Colwell's Current Methods for Classification and Identification of PDF

Biology

By Rita R. And R. Grigorova Colwell

ISBN-10: 0125215193

ISBN-13: 9780125215190

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Extra resources for Current Methods for Classification and Identification of Microorganisms

Sample text

Weak 46 B. LANYl fermenters (Haemophilus, Cardiohacterium, Flavohacterium, Kingella) produce acid slowly and frequently unreliably, even in peptone water supplemented adequately for their growth. Nutrient agar media containing glucose and lactose or glucose, lactose and sucrose are widely used in the presumptive identification of fermenters. Supplied with an acid-base indicator, these media are solidified in tubes so as to form slopes with deep butts. They usually contain thiosulphate and an iron salt for the detection of hydrogen sulphide production.

Lecithinase activity resulting in the release of fats should not be confused with the action of lipase, liberating free fatty acids from fats. Whereas the recommended substrate for lipase test is Tween 80, lipolysis can also be observed on egg-yolk agar: with oblique lighting an oily iridescent sheen (“pearly layer”) appears over the growth and in its close vicinity on the surface of the medium (Clostridium sporogenes). Fatty acids responsible for the pearly layer are stained bluish-green if the plate is flooded with saturated copper sulphate solution, the excess solution is removed and the plate is dried at 37°C for 20min (Cowan, 1974).

Suhstrute. Tween 80 sterilized at 121 "C for 20 min. lest medium. Cool the basal medium to 45-50 '(2, add Tween 80 to give a final concentration of 1 % and pour plates. (ii) Procedure. Inoculate the cultures as lines on the surface. Incubate for up to 7 days, inspect daily. (iii) Interpvctation. Positive: opaque halo around the growth (Pseudumnnus ueruginosa). Negative: halo absent (Bordetellu hronchisepticu). 15. Lecithinuse test Phospholipid complexes are emulsifying agents occurring in tissues, serum and egg yolk.

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Current Methods for Classification and Identification of Microorganisms by Rita R. And R. Grigorova Colwell


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