Get Biological Aging: Methods and Protocols (Methods in PDF
By Trygve O. Tollefsbol
This publication investigates a number of the techniques which are suffering from the age of an organism. a number of new instruments for the research of organic getting older were brought lately, and this quantity offers tools and protocols for those new thoughts as well as its insurance of tested methods. Researchers looking new expertise and strategies will locate this quantity of large gain as they circulation in the direction of new instructions.
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Extra info for Biological Aging: Methods and Protocols (Methods in Molecular Biology Vol 371)
In 1990, Seshadri and Campisi (25) demonstrated a loss of c-fos inducibility in senescent WI-38 cells, suggesting that lack of proliferation was in some part due to selective repression of c-fos. Staining for β-galactosidase is a technique to label senescent cells in situ and has been used in many studies to determine the amount of senescence achieved in culture (26–28). Principles and techniques for senescence-associated β-gal staining (SA-β-gal) are discussed in Chapter 4. 3. Quantification of Cellular Aging The aging process in vitro is accompanied by a loss in proliferative potential, which results in a decline in cellular replication.
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J. Clin. Invest. 113, 8–13. 7. Dimri, G. , et al. (1995) A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. USA 92, 9363–9367. 8. , Ramsey, M. , et al. (2004) Ink4a/Arf expression is a biomarker of aging. J. Clin. Invest. 114, 1299–1307. 9. , Brodie, S. , and Deng, C. X. (2003) Senescence, aging, and malignant transformation mediated by p53 in mice lacking the Brca1 fulllength isoform. Genes Dev. 17, 201-213. 10. Sun, L. , Lee, D. , et al.
Biological Aging: Methods and Protocols (Methods in Molecular Biology Vol 371) by Trygve O. Tollefsbol