Get Biological Aging: Methods and Protocols (Methods in PDF


By Trygve O. Tollefsbol

ISBN-10: 158829658X

ISBN-13: 9781588296580

ISBN-10: 1597453617

ISBN-13: 9781597453615

This publication investigates a number of the techniques which are suffering from the age of an organism. a number of new instruments for the research of organic getting older were brought lately, and this quantity offers tools and protocols for those new thoughts as well as its insurance of tested methods. Researchers looking new expertise and strategies will locate this quantity of large gain as they circulation in the direction of new instructions.

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Extra info for Biological Aging: Methods and Protocols (Methods in Molecular Biology Vol 371)

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In 1990, Seshadri and Campisi (25) demonstrated a loss of c-fos inducibility in senescent WI-38 cells, suggesting that lack of proliferation was in some part due to selective repression of c-fos. Staining for β-galactosidase is a technique to label senescent cells in situ and has been used in many studies to determine the amount of senescence achieved in culture (26–28). Principles and techniques for senescence-associated β-gal staining (SA-β-gal) are discussed in Chapter 4. 3. Quantification of Cellular Aging The aging process in vitro is accompanied by a loss in proliferative potential, which results in a decline in cellular replication.

Exp. Cell Res. 220, 186–193. 21. Chen, Q. , Bartholomew, J. , Reagan, J. , and Ames, B. N. (1998) Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts: p53 and Rb control G1 arrest but not cell replication. Biochem. J. 332( Pt 1), 43–50. 22. , de Oliveira, R. , Conboy, C. , and Guarente, L. (2003) Superoxide dismutase 1 knock-down induces senescence in human fibroblasts. J. Biol. Chem. 278, 38,966–38,969. 23. , Lin, A. , McCurrach, M. , and Lowe, S. W. (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a.

J. Clin. Invest. 113, 8–13. 7. Dimri, G. , et al. (1995) A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. USA 92, 9363–9367. 8. , Ramsey, M. , et al. (2004) Ink4a/Arf expression is a biomarker of aging. J. Clin. Invest. 114, 1299–1307. 9. , Brodie, S. , and Deng, C. X. (2003) Senescence, aging, and malignant transformation mediated by p53 in mice lacking the Brca1 fulllength isoform. Genes Dev. 17, 201-213. 10. Sun, L. , Lee, D. , et al.

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Biological Aging: Methods and Protocols (Methods in Molecular Biology Vol 371) by Trygve O. Tollefsbol

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