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Sample text

D. Miniaturization in analytical separation techniques generally provides several advantages such as decrease in sample injection amount, and the enhancement of separation speed, efficiency, and detection limit. D. 39 A multiplexed (Mx) HF5 device has been made with six parallel HF5 modules connected to seven-port manifolds. This assembly shows the possibility of handling up to 50 mg of proteins without incurring overloading. Mx HF5 offers a unique advantage of scaling up HF5 separation without using wide-bore HF membranes, which would increase separation time.

Counter-terrorism efforts are concerned with detecting bacterial species in as close to real-time as possible. The organism must be detected without culture Mass Spectrometry and Tandem Mass Spectrometry for Protein Biomarker Discovery 39 from an environmental or clinical sample. Many samples collected for the purpose of biodetection are usually gathered through air sampling. The problem with this type of sampling is that many bacterial species can be present in these samples creating a large amount of background during analysis.

Comparison of the signals Fig. 8 HF5 of E. coli. (1) Fimbriated strain C55 0398. (2) Non-fimbriated strain XC113 A2. 125% w/v TRIS. 44 mL/min. PSf HF channel. (Adapted with permission from ref. [31], © 2003, Elsevier Publishing) 30 P. Reschiglian et al. Table 1 Database of most characteristic MALDITOF-MS signals found with run-to-run and day-to-day variations of less than ca. 5 m/z units E. coli B.  9 MALDI/TOF m/z spectrum of a 1:1 mixture of lyophilized E. coli and B. subtilis cells. #: Most characteristic E.

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