Read e-book online RNA Interference from Biology to Therapeutics PDF
By Jesper B. Bramsen, Thomas B. Hansen (auth.), Kenneth A. Howard (eds.)
The huge, immense capability of siRNA as a healing has ended in an explosion of curiosity from the clinical neighborhood. there was severe curiosity from enormous Pharma to capitalise in this new expertise however the truth continues to be that supply is a key determinant in figuring out the complete scientific capability of RNA interference. there's an pressing want for larger supply easy methods to take this expertise ahead. This e-book addresses the function of other RNAi molecules in mobile techniques as rational for diagnostic and healing methods. This publication will disguise RNAi healing layout to optimize siRNA efficiency and decrease off-target results and present supply applied sciences to beat either intracellular and extracellular limitations. The reader will achieve an perception into RNA interference from the mobile mechanisms to screening to siRNA layout all the way through to diagnostic and healing applications.
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Extra resources for RNA Interference from Biology to Therapeutics
Morris KV (2009) RNA-directed transcriptional gene silencing and activation in human cells. Oligonucleotides 19:299–306 1 RNA Interference Pathways and Therapeutic Exploitation 29 236. Beisel C, Paro R (2011) Silencing chromatin: comparing modes and mechanisms. Nat Rev Genet 12:123–135 237. Goodchild J (2011) Therapeutic oligonucleotides. Methods Mol Biol 764:1–15 238. Crooke ST (1998) Vitravene–another piece in the mosaic. Antisense Nucleic Acid Drug Dev 8:vii–viii 239. van Deutekom JC, Janson AA, Ginjaar IB, Frankhuizen WS, Aartsma-Rus A, Bremmer-Bout M, den Dunnen JT, Koop K, van der Kooi AJ, Goemans NM et al (2007) Local dystrophin restoration with antisense oligonucleotide PRO051.
Rodriguez A, Grifﬁths-Jones S, Ashurst JL, Bradley A (2004) Identiﬁcation of mammalian microRNA host genes and transcription units. Genome Res 14:1902–1910 25. Cai X, Hagedorn CH, Cullen BR (2004) Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10:1957–1966 26. Gregory RI, Yan KP, Amuthan G, Chendrimada T, Doratotaj B, Cooch N, Shiekhattar R (2004) The Microprocessor complex mediates the genesis of microRNAs. Nature 432:235–240 27. Denli AM, Tops BB, Plasterk RH, Ketting RF, Hannon GJ (2004) Processing of primary microRNAs by the Microprocessor complex.
Nat Genet 39:914–921 224. Zeng Y, Wagner EJ, Cullen BR (2002) Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol Cell 9:1327–1333 225. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ (2005) A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci U S A 102:13212–13217 226. Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155.
RNA Interference from Biology to Therapeutics by Jesper B. Bramsen, Thomas B. Hansen (auth.), Kenneth A. Howard (eds.)