Download e-book for kindle: Regulators and Effectors of Small GTPases, Part G: Ras by W. E. Balch
By W. E. Balch
The seriously acclaimed laboratory general for greater than forty years, tools in Enzymology is without doubt one of the such a lot hugely revered guides within the box of biochemistry. considering that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless appropriate today-truly a vital booklet for researchers in all fields of lifestyles sciences. This quantity and its partners (Volumes 255, 256, 257, and the drawing close 325, 329, and 332) conceal all biochemical and organic assays presently in use for studying the function of small GTPases within the above-mentioned elements of telephone biology on the molecular point.
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Additional info for Regulators and Effectors of Small GTPases, Part G: Ras Family II
V. Lai, G. Superti-Furga, T. Pawson, P. P. Di Fiore, L. Lanfrancone, and P. G. Pelicci, EMBO J. 16, 706 (1997). 14 S. Okada, A. W. Kao, B. P. Ceresa, P. Blaikie, B. Margolis, and J. E. Pessin, J. Biol. Chem. 272, 28042 (1997). 6 CYTOPLASMIC AND NUCLEAR SIGNALING ANALYSES  FIG. 2. Specificity of antibodies to Shc family of proteins. 5 l of serum per immunoprecipitation). Immunoprecipitates were fractionated on an SDS–8% (w/v) polyacrylamide gel (Novex, San Diego, CA) and then analyzed by Western blot with the same antibodies.
Li, A. Batzer, S. Thomas, J. Brugge, P. G. Pelicci, J. Schlessinger, and T. Pawson, Nature (London) 360, 689 (1992). 17 G. Clark, A. D. Cox, S. M. Graham, and C. J. Der, Methods Enzymol. 255, 395 (1995). 18 W. S. Pear, G. P. Nolan, M. L. Scott, and D. Baltimore, Proc. Natl. Acad. Sci. A. 90, 8392 (1993). 16 8 CYTOPLASMIC AND NUCLEAR SIGNALING ANALYSES  serum. Medium may also be supplemented with penicillin (100 U/ml) and streptomycin (100 g/ml) to prevent bacterial contamination. Cells are maintained at 37◦ in 10% CO2.
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Regulators and Effectors of Small GTPases, Part G: Ras Family II by W. E. Balch