Download e-book for kindle: Regulators and Effectors of Small GTPases, Part G: Ras by W. E. Balch


By W. E. Balch

ISBN-10: 0080522564

ISBN-13: 9780080522562

ISBN-10: 0121822346

ISBN-13: 9780121822347

The seriously acclaimed laboratory general for greater than forty years, tools in Enzymology is without doubt one of the such a lot hugely revered guides within the box of biochemistry. considering that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless appropriate today-truly a vital booklet for researchers in all fields of lifestyles sciences. This quantity and its partners (Volumes 255, 256, 257, and the drawing close 325, 329, and 332) conceal all biochemical and organic assays presently in use for studying the function of small GTPases within the above-mentioned elements of telephone biology on the molecular point.

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Additional info for Regulators and Effectors of Small GTPases, Part G: Ras Family II

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V. Lai, G. Superti-Furga, T. Pawson, P. P. Di Fiore, L. Lanfrancone, and P. G. Pelicci, EMBO J. 16, 706 (1997). 14 S. Okada, A. W. Kao, B. P. Ceresa, P. Blaikie, B. Margolis, and J. E. Pessin, J. Biol. Chem. 272, 28042 (1997). 6 CYTOPLASMIC AND NUCLEAR SIGNALING ANALYSES [1] FIG. 2. Specificity of antibodies to Shc family of proteins. 5 ␮l of serum per immunoprecipitation). Immunoprecipitates were fractionated on an SDS–8% (w/v) polyacrylamide gel (Novex, San Diego, CA) and then analyzed by Western blot with the same antibodies.

Li, A. Batzer, S. Thomas, J. Brugge, P. G. Pelicci, J. Schlessinger, and T. Pawson, Nature (London) 360, 689 (1992). 17 G. Clark, A. D. Cox, S. M. Graham, and C. J. Der, Methods Enzymol. 255, 395 (1995). 18 W. S. Pear, G. P. Nolan, M. L. Scott, and D. Baltimore, Proc. Natl. Acad. Sci. A. 90, 8392 (1993). 16 8 CYTOPLASMIC AND NUCLEAR SIGNALING ANALYSES [1] serum. Medium may also be supplemented with penicillin (100 U/ml) and streptomycin (100 ␮g/ml) to prevent bacterial contamination. Cells are maintained at 37◦ in 10% CO2.

C. LEE AND REIKO T. LEE VOLUME 248. Proteolytic Enzymes: Aspartic and Metallo Peptidases Edited by ALAN J. BARRETT METHODS IN ENZYMOLOGY xxxi VOLUME 249. Enzyme Kinetics and Mechanism (Part D: Developments in Enzyme Dynamics) Edited by DANIEL L. PURICH VOLUME 250. Lipid Modifications of Proteins Edited by PATRICK J. CASEY AND JANICE E. BUSS VOLUME 251. Biothiols (Part A: Monothiols and Dithiols, Protein Thiols, and Thiyl Radicals) Edited by LESTER PACKER VOLUME 252. Biothiols (Part B: Glutathione and Thioredoxin; Thiols in Signal Transduction and Gene Regulation) Edited by LESTER PACKER VOLUME 253.

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Regulators and Effectors of Small GTPases, Part G: Ras Family II by W. E. Balch

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