Molecular Biology of Photosynthesis by Kazuo Shinozaki, Nobuaki Hayashida, Masahiro Sugiura PDF


By Kazuo Shinozaki, Nobuaki Hayashida, Masahiro Sugiura (auth.), Govindjee (eds.)

ISBN-10: 9400922698

ISBN-13: 9789400922693

ISBN-10: 9401075174

ISBN-13: 9789401075176

Molecular biology, relatively molecular genetics, is without doubt one of the most up-to-date and strongest technique in glossy photosynthesis examine. improvement of molecular biology recommendations has supplied new the way to resolve previous difficulties in lots of organic disciplines. Molecular biology has its maximum power for contribution while utilized together with different disciplines, to concentration not only on genes and molecules, yet at the complicated interplay among them and the biochemical pathways within the entire organism. Photosynthesis is definitely the simplest studied study sector in plant biology, making this box the most important candidate for effectively utilizing molecular genetic suggestions. Already, the luck of molecular biology in photosynthesis has been not anything wanting magnificent. paintings played during the last few years, a lot of that is sum­ marized during this quantity, stands in facts. concepts comparable to site-specific mutagenesis have helped us in studying the jobs of person protein domain names within the functionality of multiunit complexes equivalent to the enzyme ribulose-l ,5-bisphos­ phate carboxylase/oxygenase (RUBISCO) and the oxygen evolving picture­ approach (the photosystem II). The recommendations of molecular biology were vitally important in advancing the country of information of the response heart from the photosynthetic micro organism whose constitution has been elegantly deduced by means of H. Michel and 1. Deisenhofer from the X-ray reviews of its crystals.

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1984). The 32-kd protein is turned over rapidly and present only in very small amounts so that it has been difficult to study its structure. However, nucleotide sequence analyses of psbA enable us to deduce its protein structure. The coding region of psbA is now thought to contain 317 codons (Fig. 4) (Marder et al. 1984, Eyal et al. 1987). The 34-kd precursor polypeptide is synthesized from the psbA mRNA and seems to be processed at its C-terminal end to produce the mature 32-kd protein. The 32-kd protein is now called D1 protein and with D2 and cytochrome b559 proteins is thought to constitute the PSII reaction center (Nanba and Satoh 1987).

The intron-exon boundary sequences of petB and petD. From Tanaka et al. (1987) with minor modifications. Photosystem II complex At least 9 components of the PSII complex are encoded in N. tabacum chloroplast DNA (Table 2). The 33-kd, 24-kd and 18-kd polypeptides which function as the water-splitting apparatus are encoded in the nuclear DNA and their cDNAs have been cloned (Tittgen et al. 1986). Tobacco chloroplast genes for PSII components are located at six distinct sites in LSC (Fig. 2). A gene for the tobacco 32-kd thylakoid membrane protein (psbA) was first sequenced in N.

Chloroplast Biogenesis, pp 137-163. Cambridge: Cambridge University Press Gruissem Wand Zurawski G (I 985a) Identification and mutational analysis of the promoter for a spinach chloroplast transfer RNA gene. EMBO J 4: 1637-1644 Gruissem Wand Zurawski G (I 985b) Analysis of promoter regions for the spinach chloroplast rbeL, and alpB and psbA genes. EMBO 1 4: 3375-3383 Hallick RB and Bottomley W (1983) Proposals for the naming of chloroplast genes. Plant Mol BioI Rep I: 38-43 Hallick RB, Gingrich 1C, lohanningmeier U and Passavant CW (1985) Introns in Euglena and Nicotiana chloroplast protein genes.

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Molecular Biology of Photosynthesis by Kazuo Shinozaki, Nobuaki Hayashida, Masahiro Sugiura (auth.), Govindjee (eds.)

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