Shmuel Razin's Molecular and Diagnostic Procedures in Mycoplasmology, PDF
By Shmuel Razin
This publication and its spouse, quantity II, pay attention to new procedures--especially these in keeping with the hot molecular methodology--developed in the previous decade. This quantity outlines the methods, thoughts, and strategies utilized to mobilephone and molecular biology experiences of mycoplasmas. quantity II offers with the recent genetic and immunological instruments utilized to the prognosis of mycoplasma infections of people, animals, crops, bugs, and all cultures, with specific emphasis at the organization of mycoplasmas with the activation of AIDS. Key good points* Cultivation and morphology* Genome characterization and genetics* Membrane characterization* mobile metabolism* Taxonomy and phylogeny* Pathogenicity
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Extra resources for Molecular and Diagnostic Procedures in Mycoplasmology, Volume 1 (Molecular Procedures)
Microbiol . 138, 1785—1790 . , and Jacobs, E . (1993) . Characterization of the cellular response of spleen cells i n BALB/c mice inoculated with Mycoplasma pneumoniae or the P1 protein . Med . Microbiol. Immunol . 182, 77—85 . , and Jacobs, E . (1994) . Cytokine gene expression in the lungs of BALB/ c mice during primary and secondary intranasal infection with Mycoplasma pneumonia . Microbiology 140, 2043—2048 . Pollack, J . , Williams, M . , and McElhaney, R . N . (1995) . The comparative metabolism of the mollicutes .
C . (1994) . Induced mouse spleen B -cell proliferation and secretion o f immunoglobulin by lipid-associated membrane proteins of Mycoplasma fermentans incognitus and Mycoplasma penetrans . Infect . Immun . 62, 3916-3921 . Friedman, S . , Tumang, J . , and Crow, M . K . (1994) . A potential role for microbiol superantigens in autoimmune disease . IOM Lett. 3, 688-689 . Gundersen, D . , Rehner, S . , Davis, R . , and Kingsbury, D . T . (1994) . Phylogeny of mycoplasmalike organisms (phytoplasmas) : A basis for their classification .
Laidlawii . Chapter B 6 (this volume) describes another approach directed at the construction of shuttl e vectors capable of replication both in S . citri and in E . coli . The vectors, constructed by insertion of the S . citri origin of replication, oriC, and the tetM determinant into the colE 1-derived plasmids, could be shuttled from E . coli to S . citri and back to E. , 1994b) . On the whole, it appears that although not all the problems involved in th e development of effective shuttle vectors have been solved, the goal of efficient gene transfer among mollicutes is within reach, so that analysis of genetic mutations resulting from gene transfer among mutant strains can begin (Bove, 1993) .
Molecular and Diagnostic Procedures in Mycoplasmology, Volume 1 (Molecular Procedures) by Shmuel Razin