Karen M. Gooding, Fred E. Regnier's Libro HPLC of Biological Macromolecules PDF
By Karen M. Gooding, Fred E. Regnier
Revised and improved to mirror the newest recommendations in HPLC from the earlier decade. provides useful ideas for the assessment and research of proteins, peptides, and polynucleotides.
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Additional info for Libro HPLC of Biological Macromolecules
S. MONTENECOURT ET AL. 8 E '3-" ~ o III U ,4 ,4 0----0 l:. -I:J. 2 U DAYS Figure 9. Extracellular cellulase production by strain RUT-C30. Panel A: Growth on cellobiose (1%). Panel B: Growth on cellobiose (1%) plus glycerol (5%). Panel C: Growth on glycerol (1%). Symbols as in Figure 7. location of the in cAMP levels be reflected in cAMP must await experiments. sensor site is within nucleus. Dramatic changes may be occuring within the nucleus and would not total cell cAMP. Definitive proof of the role of demonstration of its envolvement in in vitro REGULATORY CONTROLS AND OVERPRODUCTION OF CELLULASES 47 TABLE II Activities of a Number of Enzymes of T.
Assayed according to ref. 31. a strikingly different pattern. 02 units). (Data not shown). However, substantial amounts of cellobiase activity are found in the particulate fraction during growth on glucose (Figure 6A) which is equivalent to the amount of bound activity of the cellulose grown culture (Figure 5B). Little if any cellobiase activity is found in the supernatant broth under these conditions. During growth on cellobiose (1%), a soluble but weak cellulase inducer (Figure 6B), a pattern similar to the glucose grown cultures is apparent.
E. (1977), Appl. Environ. Microbiol. li, 777. DISCUSSION Q. LADISCH: In electron micrographs of the wall were dark lines which were microfibrils, these in turn were surrounded by lignin and hemicellulose especially in certain types of wood. Do your microorganisms attack this particular lignin as well? ·E. ERIKSSON A. ERIKSSON: Yes, they do and thereby cause a swelling of the secondary wall. However, the cellulaseless mutants cannot degrade wood in a direction across the cellulose microfibrils. Q.
Libro HPLC of Biological Macromolecules by Karen M. Gooding, Fred E. Regnier