New PDF release: International Review of Cytology, Vol. 3


By G.H. Bourne, J.F. Danielli (Eds.)

ISBN-10: 0123643031

ISBN-13: 9780123643032

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Extra info for International Review of Cytology, Vol. 3

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The results with this mixture were so promising that it warranted much further study and attempts to improve upon it. With the original mixture, chick embryo tissue cultivated in roller tubes, directly on the glass without clot or other source of high molecular or unknown material, could be maintained for about 50 to 60 days. The addition of five more amino acids, and minor changes in some of the other components, resulted in a mixture (White, 1949) (Table I ) which could maintain chick embryo cells for up to 80 days, with several changes of the nutrient fluid.

In their medium. Uei (1926) reported that small amounts of Fe and Mg increased the growth of a rat sarcoma in vitro: Zn and Cu were inhibitory. Vogelaar and Erlichman (1933), and following them Baker (1936), included hemin in their media. ) would probably not add significantly to that present in other components of their media. 12 mM. of iron. 003 mM. The effects of ferric, cupric, and manganese chlorides on the survival times of chick heart cultures in biological media were studied by Hetherington and Shipp (1935).

There is, as Willmer (1941, 1942) showed, in general no correlation between high growth rate and high glucose consumption or lactic acid production. H e suggested that glucose consumption and lactic acid production are associated with cell movement, which in turn is related to the incidence of cell division. /100 mg. /day on the third and fourth days. Cultures on a schedule of daily renewal of medium, or replenishment of glucose only, used a total of 22 mg. glucose per 100 mg. /day. Of this glucose, 60 to 70% appears as lactic acid.

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International Review of Cytology, Vol. 3 by G.H. Bourne, J.F. Danielli (Eds.)

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