David A. Dansereau, Paul Lasko (auth.), Steven X. Hou, Shree's Germline Stem Cells PDF


By David A. Dansereau, Paul Lasko (auth.), Steven X. Hou, Shree Ram Singh (eds.)

ISBN-10: 1603272135

ISBN-13: 9781603272131

ISBN-10: 1603272143

ISBN-13: 9781603272148

The wisdom received via learning germline stem cells, the resource of human and animal replica, could locate speedy software in keeping endangered natural world, coping with advertisement farm animals, overcoming fertility difficulties in people, and treating testicular and ovary tumors. In Germline Stem Cells, top specialists discover the parameters that outline germline stem cells and the mechanisms that keep watch over the phone habit with the intention to greater isolate, represent and retain them. Divided into elements, the amount starts off via delivering protocols for germline stem mobilephone id and law in version organisms, and concludes with designated chapters protecting present suggestions related to in vitro tradition and the purposes of the cells. Following the hugely winning Methods in Molecular Biology™ sequence layout, every one bankruptcy features a short advent to the topic, a listing of invaluable apparatus and reagents, step by step laboratory protocols, and tips about troubleshooting and keeping off identified pitfalls.

Comprehensive and state of the art, Germline Stem Cells is the proper reference for scientists exploring this actual fascinating and significant subdivision within the fast paced box of stem cellphone research.

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1994) Primordial germ cell migration and the assembly of the Drosophila embryonic gonad. Dev. Biol. 166, 180–194. 51. , and DiNardo, S. (1997) Expression and function of clift in the development of somatic gonadal precursors within the Drosophila mesoderm. Development. 124, 971–982. 52. Broihier, H. , Moore, L. , and Lehmann, R. (1998) zfh-1 is required for germ cell migration and gonadal mesoderm development in Drosophila. Development. 125, 655–666. 53. , and O’Connor, M. (1990) Regulatory elements of the bithorax complex that control expression along the anterior-posterior axis.

Elegans germline. Dev. Cell. 7, 697–707. 22. , Lee, M. , et al. (2005) Translational repression of C. elegans p53 by GLD-1 regulates DNA damage-induced apoptosis. Cell. 120, 357–368. 23. Jones, A. , and Schedl, T. (1996) GLD-1, a cytoplasmic protein essential for oocyte differentiation, shows stage-and sex-specific expression during Caenorhabditis elegans germline development. Dev. Biol. 180, 165–183. 24. Hendzel, M. , Mancini, M. , et al. (1997) Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.

Dry in 65°C oven for approx. 30 min. Slides can be stored in the oven or at room temperature. 6. Paraformaldehyde: 16% stock (Electron Microscopy Sciences, cat. no. 15710). 7. 5% bovine serum albumin (BSA). L. Crittenden and J. Kimble 8. DAPI (4¢,6-diamidino-2-phenylindole) (Molecular Probes, Invitrogen cat. no. D1306). 9. Vectashield (Vector Labs, cat. no. H-1000). 10. Hoechst 33342 (Molecular Probes, Invitrogen cat. no. H3570). 11. SYTO-12 (Molecular Probes, Invitrogen cat. no. S7574). 12. Rabbit antiphosphohistone H3 (PH3) polyclonal antibody (Upstate Biotechnology, cat.

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Germline Stem Cells by David A. Dansereau, Paul Lasko (auth.), Steven X. Hou, Shree Ram Singh (eds.)

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