Exocytosis and Endocytosis by Elena V. Vassilieva, Asma Nusrat (auth.), Andrei I. Ivanov PDF

Biology

By Elena V. Vassilieva, Asma Nusrat (auth.), Andrei I. Ivanov (eds.)

ISBN-10: 1588298655

ISBN-13: 9781588298652

Due to their very important involvement in a large choice of home tasks and really good mobile features, exocytosis and endocytosis stay one of the most well-liked topics in biology and biomedical sciences. large development in realizing those advanced intracellular strategies has been completed by way of utilising a wide range of study instruments starting from classical biochemical ways to sleek imaging concepts. In Exocytosis and Endocytosis, expert specialists give you the such a lot up-to-date,step-by-step laboratory protocols for interpreting molecular equipment and organic features of exocytosis and endocytosis in vitro and in vivo. Following the hugely winning Methods in Molecular Biology™ sequence layout, the chapters current an creation outlining the primary in the back of every one procedure, a listing of the required fabrics, a simple to keep on with, with ease reproducible protocol, and a Notes part supplying tips about troubleshooting and fending off recognized pitfalls.

Insightful to either novices and pro execs, Exocytosis and Endocytosis deals a special and hugely functional advisor to flexible laboratory instruments constructed to check a number of points of intracellular vesicle trafficking in easy version platforms and dwelling organisms.

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Acknowledgments I thank Moshe Bachar for his excellent editorial assistance and Drs. Alex Mongin and Andrew Kowalczyk for their valuable comments on the manuscript. This work was supported by a Career Development Award from the Crohn’s and Colitis Foundation of America. References 1. L. (2003) Regulated portals of entry into the cell. Nature 422, 37–44. 2. T. (1999) The structural era of endocytosis. Science 285, 215–220. 3. A. (2003) Lipid rafts and caveolae as portals for endocytosis: new insights and common mechanisms.

Cell Res. 229, 307–317. 56. , and Just, I. (2003) Thiol-modifying phenylarsine oxide inhibits guanine nucleotide binding of Rho but not of Rac GTPases. Mol. Pharmacol. 63, 1349–1355. 57. G. (2002) Alterations in membrane cholesterol that affect structure and function of caveolae. Methods Enzymol. 353, 131–139. 58. , and Laufs, U. (2005) Pleiotropic effects of statins. Annu. Rev. Pharmacol. Toxicol. 45, 89–118. 59. A. (2003) Lovastatin and beyond: the history of the HMG-CoA reductase inhibitors.

J. Cell Biol. 109, 2731–2739. 94. , et al. (2001) Human immunodeficiency virus type 1 entry into macrophages mediated by macropinocytosis. J. Virol. 75, 11166–11177. 95. , et al. (2004) Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement. Mol. Ther. 10, 1011–1022. 96. H. (2002) The route of bacterial uptake by macrophages influences the repertoire of epitopes presented to CD4 T cells. Eur. J. Immunol. 32, 3714–3719. 97. , et al. (2006) Effects of Na+/H+ exchanger inhibitors on subcellular localisation of endocytic organelles and intracellular dynamics of protein transduction domains HIV-TAT peptide and octaarginine.

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Exocytosis and Endocytosis by Elena V. Vassilieva, Asma Nusrat (auth.), Andrei I. Ivanov (eds.)


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