Exocytosis and Endocytosis by Elena V. Vassilieva, Asma Nusrat (auth.), Andrei I. Ivanov PDF


By Elena V. Vassilieva, Asma Nusrat (auth.), Andrei I. Ivanov (eds.)

ISBN-10: 1588298655

ISBN-13: 9781588298652

Due to their very important involvement in a large choice of home tasks and really good mobile features, exocytosis and endocytosis stay one of the most well-liked topics in biology and biomedical sciences. large development in realizing those advanced intracellular strategies has been completed by way of utilising a wide range of study instruments starting from classical biochemical ways to sleek imaging concepts. In Exocytosis and Endocytosis, expert specialists give you the such a lot up-to-date,step-by-step laboratory protocols for interpreting molecular equipment and organic features of exocytosis and endocytosis in vitro and in vivo. Following the hugely winning Methods in Molecular Biology™ sequence layout, the chapters current an creation outlining the primary in the back of every one procedure, a listing of the required fabrics, a simple to keep on with, with ease reproducible protocol, and a Notes part supplying tips about troubleshooting and fending off recognized pitfalls.

Insightful to either novices and pro execs, Exocytosis and Endocytosis deals a special and hugely functional advisor to flexible laboratory instruments constructed to check a number of points of intracellular vesicle trafficking in easy version platforms and dwelling organisms.

Show description

Read or Download Exocytosis and Endocytosis PDF

Best biology books

New PDF release: Power, Sex, Suicide: Mitochondria and the Meaning of Life

If it weren't for mitochondria, scientists argue, we'd all nonetheless be single-celled micro organism. certainly, those tiny constructions inside of our cells are very important past imagining. with no mitochondria, we might don't have any mobilephone suicide, no sculpting of embryonic form, no sexes, no menopause, no aging.

In this interesting and thought-provoking booklet, Nick Lane brings jointly the most recent examine during this interesting box to teach how our starting to be perception into mitochondria has make clear how complicated lifestyles developed, why intercourse arose (why don't we simply bud? ), and why we age and die. those findings are of primary significance, either in knowing existence on the earth, but in addition in controlling our personal health problems, and delaying our degeneration and loss of life. Readers research that billion years in the past, mitochondria have been most likely micro organism dwelling autonomous lives and that their trap inside of better cells used to be a turning element within the evolution of lifestyles, allowing the improvement of complicated organisms. Lane describes how mitochondria have their very own DNA and that its genes mutate a lot speedier than these within the nucleus. This excessive mutation expense lies at the back of our getting older and likely congenital ailments. the most recent examine means that mitochondria play a key position in degenerative ailments reminiscent of melanoma. We additionally observe that mitochondrial DNA is handed down virtually solely through the feminine line. That's why it's been utilized by a few researchers to track human ancestry daughter-to-mother, to "Mitochondrial Eve," giving us important information regarding our evolutionary history.

Written by means of Nick Lane, a emerging big name in well known technology, strength, intercourse, Suicide is the 1st booklet for common readers at the nature and serve as of those tiny, but attention-grabbing buildings.

Alex Reid (auth.), Marilena Aquino de Muro, Ralph Rapley's Gene Probes: Principles and Protocols PDF

Using nucleic acid or gene probes as cloning or diagnostic instruments has promoted major advances in human genome mapping and within the isolation and manipulation of genes as a rule. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have introduced jointly a great number of time-tested protocols for designing and utilizing gene probes in a wide selection of functions.

J. S. Sussenbach, P. H. Steenbergh, E. Jansen, P.'s Molecular Biology and Physiology of Insulin and Insulin-Like PDF

This quantity addresses a basic puzzle in biology and medication, specifically, how does tissue advance, fix and change itself. the reply appears to be like to lie in development elements and their rules. To thrive and live to tell the tale we want development elements and this ebook concentrates on components which are on the topic of development hormone.

Get Serotonin: From Cell Biology to Pharmacology and PDF

This quantity represents the complaints ofthe invited lectures ofthe first foreign Symposium on "Serotonin from phone Biology to Pharmacology and Therapeutics" which was once held in Florence on March 29 -Aprill, 1989. This assembly, held lower than the co-sponsorship of the Serotonin membership and the Fondazione Giovanni Lorenzini, represents the 1st try to collect scientists thinking about the complexity of the motion of 5-hydroxytryptamine during the physique and in a number of species.

Additional resources for Exocytosis and Endocytosis

Example text

Acknowledgments I thank Moshe Bachar for his excellent editorial assistance and Drs. Alex Mongin and Andrew Kowalczyk for their valuable comments on the manuscript. This work was supported by a Career Development Award from the Crohn’s and Colitis Foundation of America. References 1. L. (2003) Regulated portals of entry into the cell. Nature 422, 37–44. 2. T. (1999) The structural era of endocytosis. Science 285, 215–220. 3. A. (2003) Lipid rafts and caveolae as portals for endocytosis: new insights and common mechanisms.

Cell Res. 229, 307–317. 56. , and Just, I. (2003) Thiol-modifying phenylarsine oxide inhibits guanine nucleotide binding of Rho but not of Rac GTPases. Mol. Pharmacol. 63, 1349–1355. 57. G. (2002) Alterations in membrane cholesterol that affect structure and function of caveolae. Methods Enzymol. 353, 131–139. 58. , and Laufs, U. (2005) Pleiotropic effects of statins. Annu. Rev. Pharmacol. Toxicol. 45, 89–118. 59. A. (2003) Lovastatin and beyond: the history of the HMG-CoA reductase inhibitors.

J. Cell Biol. 109, 2731–2739. 94. , et al. (2001) Human immunodeficiency virus type 1 entry into macrophages mediated by macropinocytosis. J. Virol. 75, 11166–11177. 95. , et al. (2004) Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement. Mol. Ther. 10, 1011–1022. 96. H. (2002) The route of bacterial uptake by macrophages influences the repertoire of epitopes presented to CD4 T cells. Eur. J. Immunol. 32, 3714–3719. 97. , et al. (2006) Effects of Na+/H+ exchanger inhibitors on subcellular localisation of endocytic organelles and intracellular dynamics of protein transduction domains HIV-TAT peptide and octaarginine.

Download PDF sample

Exocytosis and Endocytosis by Elena V. Vassilieva, Asma Nusrat (auth.), Andrei I. Ivanov (eds.)

by Donald

Rated 4.40 of 5 – based on 28 votes