Download e-book for kindle: Electron Microscopy: Methods and Protocols by John J. Bozzola (auth.), John Kuo (eds.)


By John J. Bozzola (auth.), John Kuo (eds.)

ISBN-10: 1588295737

ISBN-13: 9781588295736

ISBN-10: 1597452947

ISBN-13: 9781597452946

The revised and elevated moment version of this publication offers the most recent know-how in electron microscopy, whereas conserving the practicality and accessibility of the acclaimed first version. just like the first version, this quantity offers transparent, concise directions on processing organic specimens and contains dialogue at the underlying rules of nearly all of the techniques awarded. Electron Microscopy includes significant parts of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM region covers a number of key options, together with: traditional specimen education tools for aesthetic cells and biomedical and plant tissues; cryospecimen guidance through high-pressure freezing and cryoultramicrotomy adverse staining and immunogold labeling concepts; and TEM crystallography and cryo-TEM tomography. The SEM zone equally attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy strategies, in addition to the applying of X-ray microanalysis. Protocols for the appliance of X-ray microanalysis to SEM and mass spectrometry finish the volume.

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Additional resources for Electron Microscopy: Methods and Protocols

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This chemical is highly toxic to mucous membranes and eyes. Work in hood and avoid vapors. Osmium tetroxide is used as a secondary fixative that is similarly buffered with sodium phosphate. Stock 2% osmium tetroxide is prepared by dissolving 1 gram of crystalline osmium tetroxide in 50 mL of distilled water. The fixative is prepared as follows: 1. 1 M phosphate buffer. 3. 1. 2 M dual sodium solution is prepared from a combination of anhydrous dibasic sodium phosphate (Na2HPO4) and monobasic sodium phosphate (NaH2PO4 .

To open up plastic culture flasks, melt a series of holes in the upper side of the flask using either a soldering iron or a heated glass rod. Do this in a fume hood as noxious fumes are generated. Place the open culture vessels on top of a warm spot, such as an incubator, as this will speed the evaporation of ethanol. 12. Polymerized epoxy plastics can be stripped from conventional plastic Petri dishes relatively easily with a pair of pliers. Immediately after removing the plastic Petri dish from the oven, use the pliers to remove all polystyrene making up the wall of the plastic dish, leaving only the bottom of the plate with the overlying layer of epoxy.

1977) The use of PIPES buffer in the fixation of mammalian and marine tissues for electron microscopy. J. Microsc. (Oxford) 109, 315–321. 7. Edwards, H. , Yeh, Y. , Tarnowsky, B. , and Schonbaum, G. R. (1992) Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: Comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Microsc. Res. Tech. 21, 39–50. 8. Ryter, A. and Kellenberger, E. (1958) Etude au microscope electronique de plasma contenant de l’acide desoxyribonucleique.

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Electron Microscopy: Methods and Protocols by John J. Bozzola (auth.), John Kuo (eds.)

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