Read e-book online Cell-Based Assays for High-Throughput Screening: Methods and PDF
By W. Frank An, Nicola J. Tolliday (auth.), Paul A. Clemons, Nicola J. Tolliday, Bridget K. Wagner (eds.)
As using high-throughput screening expands and creates extra curiosity within the educational group, the necessity for special reference fabrics turns into ever extra urgent. Cell-Based Assays for High-Throughput Screening: equipment and Protocols goals to fill an immense a part of this desire through supplying an simply obtainable reference quantity for cell-based phenotypic screening. top researchers within the box give a contribution cutting-edge equipment with actionable protocols overlaying 4 significant parts of analysis: version organic structures, screening modalities and assay platforms, detection applied sciences, and ways to facts research. Written within the hugely winning Methods in Molecular Biology™ sequence structure, every one bankruptcy encompasses a short creation to the topic, lists of helpful fabrics and reagents, step by step laboratory protocols, and a Notes part detailing pointers on troubleshooting and warding off identified pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: equipment and Protocols offers an outline of suitable techniques, allowing the direct program of current easy methods to new discoveries whereas additionally inspiring researchers to method their screening tasks in a conceptually modular style, improving the ability to find via new mixtures of current approaches.
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Extra resources for Cell-Based Assays for High-Throughput Screening: Methods and Protocols
Cover each source plate containing small molecule library with an aluminum sealing tape. Ensure that the tape forms a tight seal using a rolling sealer. Put the source plates back in a –80°C freezer for long-term storage. 4. Screening for Effects of Small Molecules by Visual Inspection of Phenotypes 1. ” 2. At desired times in development, remove the 96-well plates containing compound-treated embryos from the incubator and examine each well under a stereomicroscope. For better visualization of subtle changes, such as changes in circulatory pattern, a phase-contrast inverted microscope can be used.
High-throughput cell-based assays have proven to be powerful tools to deconvolute complex signaling pathways, including the MAP kinase pathways. For many decades, in vitro culture of mammalian cells has been harnessed to study basic biological questions. Diverse transformed and primary mammalian cells have been exploited to study diseases, including diabetes, cancer, and neurodegenerative disease (7, 8). However, many breakthroughs have depended on the serendipitous discovery of a small-molecule inhibitor with specificity for a particular biological process.
And Pour, P. M. (2002) Pancreatic cell lines: A review. Pancreas 24, 111–120. 9. , Yamamoto, T. and Nishida, E. (2004) Regulatory mechanisms and function of ERK MAP kinases. J. Biochem. (Tokyo) 136, 557–561. 10. Yoon, S. and Seger, R. (2006) The extracellular signal-regulated kinase: Multiple substrates regulate diverse cellular functions. Growth Factors 24, 21–44. 41 11. Gimbrone Jr, M. , Cotran, R. S. and Folkman, J. (1974) Human vascular endothelial cells in culture: Growth and DNA synthesis.
Cell-Based Assays for High-Throughput Screening: Methods and Protocols by W. Frank An, Nicola J. Tolliday (auth.), Paul A. Clemons, Nicola J. Tolliday, Bridget K. Wagner (eds.)