Bioconjugation Protocols: Strategies and Methods (Methods in by Christof M. Niemeyer PDF

Biology

By Christof M. Niemeyer

ISBN-10: 1588290980

ISBN-13: 9781588290984

ISBN-10: 1592598137

ISBN-13: 9781592598137

Professional laboratorians replace the vintage bioconjugation equipment and introduce worthy new suggestions that transcend natural biomedical functions to incorporate parts from complicated natural synthesis, molecular biology, and fabrics technology. those with ease reproducible equipment disguise the education of protein conjugates utilizing covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates utilizing various labeling innovations, and techniques to semisynthetic conjugates of proteins. extra chapters tackle the biofunctionalization of inorganic surfaces, together with the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput research of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic strength microscopy.

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Additional resources for Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology Vol 283)

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158, 773–785. Internalization and Pulmonary Targeting 21 2 Characterization of Endothelial Internalization and Targeting of Antibody–Enzyme Conjugates in Cell Cultures and in Laboratory Animals Silvia Muro, Vladimir R. Muzykantov, and Juan-Carlos Murciano Summary Streptavidin–biotin conjugates of enzymes with carrier antibodies provide a versatile means for targeting selected cellular populations in cell cultures and in vivo. Both specific delivery to cells and proper subcellular addressing of enzyme cargoes are important parameters of targeting.

2003) Immunotargeting of catalase to the pulmonary endothelium alleviates oxidative stress and reduces acute lung transplantation injury. Nat. Biotechnol. 21, 392–398. 12. Wilchek, M. and Bayer, E. , eds. (1990) Avidin-Biotin Technology. , San Diego, CA. 13. Odian, G. (1991) Principles of Polymerization. , New York, NY. 14. , and Fujiwara, K. (2002) Evidence for a role of platelet endothelial cell adhesion molecule-1 in endothelial cell mechanosignal transduction: is it a mechanoresponsive molecule?

Med. 34, 1035–1046. 10. 10 Murciano, J. , Harshaw, D. , Albelda, S. , et al. (2003) ICAM-directed vascular immunotargeting of anti-thrombotic agents to the endothelial luminal surface. Blood, 101, 3977–3984. 11. 11 Kozower, B. , Sweitzer, T. , Buerk, D. , Solomides, C. , et al. (2003) Immunotargeting of catalase to the pulmonary endothelium alleviates oxidative stress and reduces acute lung transplantation injury. Nat. Biotechnol. 21, 392–398. 12. Wilchek, M. and Bayer, E. , eds. (1990) Avidin-Biotin Technology.

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Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology Vol 283) by Christof M. Niemeyer


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