Benign by Design. Alternative Synthetic Design for Pollution - download pdf or read online
By Paul T. Anastas, Carol A. Farris
content material: Benign by way of layout chemistry / Paul T. Anastas --
Environmentally benign chemical synthesis and processing for the financial system and the surroundings / Kenneth G. Hancock and Margaret A. Cavanaugh --
Microbial biocatalysis : synthesis of adipic acid from D-glucose / Karen M. Draths and John W. Frost --
Mechanistic research of a catalytic procedure for carbonylation of nitroaromatic compounds : constructing possible choices to be used of phosgene / Wayne L. Gladfelter and Jerry D. Gargulak --
Preparative reactions utilizing obvious mild : excessive yields from pseudoelectrochemical transformation / Gary A. Epling and Qingxi Wang --
A photochemical replacement to the Friedel-Crafts response / George A. Kraus, Masayuki Kirihara, and Yusheng Wu --
Mn(III)-mediated electrochemical oxidative free-radical cyclizations / Barry B. Snider and Bridget A. McCarthy --
Supercritical carbon dioxide as a medium for undertaking free-radical reactions / James M. Tanko, Joseph F. Blackert, and Mitra Sadeghipour --
The college of California-Los Angeles styrene method / Orville L. Chapman --
iteration of urethanes and isocyanates from amines and carbon dioxide / Dennis Riley, William D. McGhee, and Thomas Waldman --
Nucleophilic fragrant substitution for hydrogen : new halide-free routes for construction of fragrant amines / Michael ok. Stern --
Chemistry and catalysis : keys to environmentally more secure procedures / Leo E. Manzer --
replacement syntheses and different resource relief possibilities for premanufacture notification components on the U.S. Environmental security corporation / Carol A. Farris, Harold E. Podall, and Paul T. Anastas --
Computer-assisted replacement man made layout for pollutants prevention on the U.S. Environmental safety organisation / Paul T. Anastas, J. Dirk Nies, and Stephen C. DeVito.
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Extra resources for Benign by Design. Alternative Synthetic Design for Pollution Prevention
Calcoaceticus was generously provided by the Ornston group. Final assembly of the microbial catalyst required amplification of three native E. coli genes including tkt, aroF, and aroB and expression of three foreign genes including aroZ and aroY from K. pneumoniae and cat A from A. calcoaceticus. To ensure plasmid compatibility and plasmid maintenance, three vectors possessing compatible replication origins and each encoding resistance to a different antibiotic were identified. Vector pBR325 (53) contains the pMBl origin of replication.
A mutation in the gene (aroE) encoding shikimate dehydrogenase results in accumulation of DHS, the substrate of the missing enzyme, in the culture supernatant. Analysis of the culture supernatant of E. coli AB2834 aroE by proton nuclear magnetic resonance (*H NMR) indicated that this strain synthesized 9 mM DHS from 56 mM D-glucose (43). Since no other common pathway metabolites were detected in the culture supernatant, it was concluded that all the D-glucose directed into aromatic biosynthesis was converted into DHS.
DRATHS& FROST Microbial Biocatalysis 41 aromatic catabolism. In various fungi and bacteria, DHS dehydratase plays a critical role in allowing an organism to metabolize hydroaromatics such as shikimic acid as a sole carbon source. Conversion of DHS into protocatechuic acid catalyzed by DHS dehydratase yields a substrate which can be further metabolized via the p-hydroxybenzoate branch of the β-ketoadipate pathway (46). s, c/s-muconic acid (46). In order to properly channel protocatechuic acid into cis, cw-muconate biosynthesis, protocatechuate decarboxylase (Figure 5, enzyme B) was required.
Benign by Design. Alternative Synthetic Design for Pollution Prevention by Paul T. Anastas, Carol A. Farris