Agrobacterium Protocols (Methods in Molecular Biology Vol - download pdf or read online
By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols bargains starting and skilled researchers the main complete number of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the subjects variety from the upkeep of bacterial tradition collections to elements of the metabolism and body structure of reworked tissues and transgenic crops. Drawing at the paintings of major scientists from laboratories worldwide, Agrobacterium Protocols presents a wealth of ideas for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered vegetation. Its distinct techniques will facilitate speedy move of complex suggestions to different laboratories and their exploitation in basic and utilized plant biology.
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Extra info for Agrobacterium Protocols (Methods in Molecular Biology Vol 44)
J and Schilperoort, R. A. (1992) Agrobacterium and plant genetic engineering. Plant Mol. Biol. 19,15-38. , and Schell, J. (1983) Ti plasmrd vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacrty. EMBO J 2,2143-2150. 5. , Murphy, E. B , Boberts, J L , Gustafson, G. , and Malcolm, S. K (1985) Resistance to hygromycin B A new marker for plant transformation studres. Plant Mol Biol 5, 103-108. 6 Holt, J. , Powles, S. , and Holtum, A M. (1993) Mechanisms and agronomic aspect of herbicide resistance.
Samson, Havana 425 Petunia hybrida (Petunia) cv Mrtchell Phaseolus vulgaris (Green bean) cv. Dark Red Kidney Solanum melongena (Aubergine) cv. Ptcentia Solanum tuberosum (Potato) line NDD-277-2 20 7 21 22 I1 10 6 23 24 4 25 26 27 28 typically normal and able to grow on their own roots (1,2). The development of small binary vectors, in which the T-DNA is separated on an independent replicon from the remainder of the Ti or Ri plasmid, facilitated foreign gene insertion between the T-DNA borders (I).
In some strains of A. tumefaciens, exposure to these inducing conditions leads to inhibition of bacterial growth, which can then be followed by the selection of avirulent mutants (I). Observation of this effect of vir gene induction depends on the use of particular experimental conditions. This chapter first specifies experimental parameters allowing to reveal, in A. tumefaciens, the modifications that accompany vir gene induction. Other parts of the chapter then describe how the mutants that are generated in the presence of acetosyringone are recognized and characterized.
Agrobacterium Protocols (Methods in Molecular Biology Vol 44) by Kevan M. A. Gartland, Michael R. Davey